Production of Recombinant Proteins in Transgenic Tobacco Plants

Tim H Szeto; Pascal M W Drake; Audrey Y-H Teh; Nicole Falci Finardi; Ashleigh G Clegg; Mathew J Paul; Rajko Reljic; Julian K-C Ma

doi:10.1007/978-1-0716-2241-4_2

Production of Recombinant Proteins in Transgenic Tobacco Plants

Methods Mol Biol. 2022:2480:17-48. doi: 10.1007/978-1-0716-2241-4_2.

Authors

Affiliations

¹ Hotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK.
² Hotung Molecular Immunology Unit, St. George's University of London, Institute for Infection and Immunity, London, UK. pdrake@sgul.ac.uk.

^# Contributed equally.

PMID: 35616855
DOI: 10.1007/978-1-0716-2241-4_2

Abstract

Nicotiana tabacum (the tobacco plant ) has numerous advantages for molecular farming, including rapid growth, large biomass and the possibility of both cross- and self-fertilization. In addition, genetic transformation and tissue culture protocols for regeneration of transgenic plants are well-established. Here, we describe the production of transgenic tobacco using Agrobacterium tumefaciens and the analysis of recombinant proteins, either in crude plant extracts or after purification, by enzyme-linked immunosorbent assays, sodium dodecyl sulfate polyacrylamide gel electrophoresis with western blotting and surface plasmon resonance.

Keywords: Agrobacterium tumefaciens; ELISA; Nicotiana tabacum; Recombinant protein; SDS PAGE; Surface plasmon resonance; Transgenic; Western blotting.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium tumefaciens* / genetics
Agrobacterium tumefaciens* / metabolism
Blotting, Western
Nicotiana* / metabolism
Plants, Genetically Modified
Recombinant Proteins / metabolism

Substances

Recombinant Proteins