Novel role of Dipterocarpus tuberculatus as a stimulator of focal cell adhesion through the regulation of MLC2/FAK/Akt signaling pathway

Cell Adh Migr. 2022 Dec;16(1):72-93. doi: 10.1080/19336918.2022.2073002.

Abstract

To investigate a novel function of Dipterocarpus tuberculatus on focal cell adhesion stimulation, alterations to the regulation of focal cell adhesion-related factors were analyzed in NHDF cells and a calvarial defect rat model after treatment with methanol extracts of D. tuberculatus (MED). MED contained gallic acid, caffeic acid, ellagic acid, and naringenin in high concentrations. The proliferation activity, focal cell adhesion ability, adhesion receptors-mediated signaling pathway in NHDF cells were increased by MED. Also, a dense adhered tissue layer and adherent cells on MED-coated titanium plate (MEDTiP) surfaces were detected during regeneration of calvarial bone. The results of the present study provide novel evidence that MED may stimulate focal cell adhesion in NHDF cells and a calvarial defect rat model.

Keywords: Dipterocarpus tuberculatus; FAK; PI3K; focal cell adhesion; integrin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Dipterocarpaceae* / chemistry
  • Focal Adhesion Protein-Tyrosine Kinases
  • Focal Adhesions
  • Myosin Light Chains
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt*
  • Rats
  • Signal Transduction*

Substances

  • Myosin Light Chains
  • Focal Adhesion Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins c-akt

Grants and funding

This work was funded by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT), grant number 2019R1A2C1084140. This study was supported by the BK21 FOUR Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Korea, grant number F21YY8109033 and F22YY8109033.