Knockout of circRNA single stranded interacting protein 1 (circRBMS1) played a protective role in myocardial ischemia-reperfusion injury though inhibition of miR-2355-3p/Mammalian Sterile20-like kinase 1 (MST1) axis

Bioengineered. 2022 May;13(5):12726-12737. doi: 10.1080/21655979.2022.2068896.

Abstract

Evidence suggests circRBMS1 regulates mRNA to mediate cell apoptosis, inflammation, and oxidative stress in different diseases. MST1 is reported to be the target and activator of apoptosis-related molecules and signaling pathways. Hence, the present study aims to investigate the role of circ-RBMS1/miR-2355-3p/MST1 in the development of I/R injury. In vitro experiments showed increased circ-RBMS1 and decreased miR-2355-3p in H/R-induced HCMs. CircRBMS1 served as a sponge for miR-2355-3p and miR-2355-3p targeted MST1. Furthermore, knockout of circRBMS1 attenuated cell apoptosis, oxidized stress, and inflammation in H/R-induced HCMs. In vivo experiments indicated circRBMS1 knockdown attenuated cardiac function damage, cell apoptosis, oxidative stress injury and inflammatory response through miR-2355-3p/MST1 axis in mice. In summary, these results demonstrated circRBMS1 played a protective role in myocardial I/R injury though inhibition of miR-2355-3p/MST1 axis. It might provide a new therapeutic target for cardiac I/R injury.

Keywords: MST1; Myocardial I/R injury; circRBMS1; miR-2355-3p.

MeSH terms

  • Animals
  • Apoptosis / genetics
  • Inflammation / metabolism
  • Mice
  • Mice, Knockout
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Myocardial Reperfusion Injury* / genetics
  • Myocardial Reperfusion Injury* / metabolism
  • Myocytes, Cardiac / metabolism
  • Protein Serine-Threonine Kinases* / genetics
  • RNA, Circular* / genetics

Substances

  • MicroRNAs
  • RNA, Circular
  • Stk4 protein, mouse
  • Protein Serine-Threonine Kinases

Grants and funding

This study was funded by Natural Science Foundation of Jiangxi Province (20202BABL206070), Primary Research & Developement Plan of Jiangxi Province (20212BBG73020) and National Natural Science Foundation of China (82060344).