Wheat embryo albumin (WEA) extracted from wheat embryo possesses multiple effects including antioxidant, anti-inflammatory, and immunoregulatory effects. In this study, a single factor experiment was conducted to determine the optimal enzymolysis conditions of WEA. Five components (F1-F5) were obtained via ultrafiltration, among which F3 (molecular weight 3-5 kDa) displayed the best antioxidant activity. WEA and F3 were characterized by transmission electron microscopy, scanning electron microscopy, circular dichroism spectrum analysis, and amino acid composition tests. Results revealed that F3 significantly increased the contents of β-tablets, aromatic amino acids, and hydrophobic amino acids compared to WEA. LC-MS/MS analysis demonstrated that F3 had more tyrosine and histidine moieties than WEA. Moreover, analysis of the Maillard reaction products (MRPs) showed that F3-MRPs had strong browning strength, ultraviolet absorption, higher number of free amino acids, and umami amino acid ratio compared with WEA. In conclusion, enzymolysis can improve the functional properties of WEA, which broadens the application spectrum of WEA in food and pharmaceutical fields. PRACTICAL APPLICATION: This study provides a new approach for identifying potential antioxidants and developing functional foods from WEA, and broadens the application spectrum of wheat germ resources.
Keywords: LC-MS/MS; Maillard reaction; antioxidant peptides; physicochemical characterization; wheat embryo albumin.
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