Effects of wastewater-spiked nanoparticles of silver and titanium dioxide on survival, growth, reproduction and biochemical markers of Daphnia magna

Abstract

Silver (Ag) and titanium dioxide (TiO₂) nanoparticles (NPs) are released into aquatic environments through wastewater treatment plants (WWTPs). Even though these NPs are mostly retained in WWTPs, a small fraction can be found in released effluents and may exert toxic effects on aquatic biota. Currently, the available information about the sublethal effects of wastewater-borne NPs on aquatic organisms is inconclusive and the importance of exposure media remains poorly understood. Previously, we demonstrated that rainbow trout juveniles chronically exposed to wastewater-borne AgNPs or TiO₂NPs caused no effects on growth, but antioxidative stress mechanisms were triggered in fish organs. Accordingly, this study aimed to: (i) assess the chronic (21-d) effects of wastewater-borne AgNPs (0.3-23.5 μg L^-1 Ag) and TiO₂NPs (2.7-3.9 μg L^-1 Ti) on survival, growth and reproduction of Daphnia magna; (ii) determine the short-term (96-h) effects of wastewater-borne AgNPs (30.3 μg L^-1 Ag) and TiO₂NPs (6.3 μg L^-1 Ti) at the subcellular level (biochemical markers of neurotoxicity, anaerobic metabolism and oxidative stress); and (iii) compare the effects obtained in (i) and (ii) with the corresponding ones induced by effluent-supplemented and water-dispersed NPs. Total Ag and Ti levels were analytically quantified in all treatments. It was demonstrated that both wastewater-borne NPs are considered non-toxic to daphnids at tested concentrations, considering the endpoints at the individual (survival, growth, reproduction) and subcellular (biochemical markers) levels. Contrarily, when pristine forms of NPs were supplemented to effluents or water, concentration-dependent effects were noticed, particularly on cumulative offspring of daphnids. The significant effects on anaerobic metabolism and detoxification pathways caused by the effluent indicate background toxicity. Bearing in mind the achievement of a suitable risk assessment of NPs in aquatic environments, this combined approach looking at both the individual and subcellular levels responses come up with relevant information about the ecotoxicological harmlessness of wastewater-borne NPs in complex environmental matrices like WWTP effluents.

Keywords: AgNPs; Biomarkers; Chronic bioassay; Cladoceran; TiO(2)NPs; WWTP effluent.