Evidence for Multiple Origins of Knockdown Resistance (kdr) in Spodoptera exigua (Hübna) (Lepidoptera: Noctuidae) From China

Abstract

The beet armyworm, Spodoptera exigua (Hübna) is a serious agricultural pest that is challenging to control due to resistance to most pesticides, including pyrethroids. This resistance has previously been linked to the knockdown resistance (kdr) mutation (L1014F) of the voltage-gated sodium channel (VGSC) in S. exigua. To better understand the frequencies of the kdr mutation of SeVGSC and identify the evolutionary origins of kdr mutation in S. exigua, seven populations of S. exigua were collected in China, and partial SeVGSC genomic sequences for each individual were acquired. The bioassays showed that the survival rates of seven populations of S. exigua larvae exposed to the discriminating dose of beta-cypermethrin (0.05 mg/cm2) ranged from 91.66% to 100%, indicating that all seven populations had evolved resistance to beta-cypermethrin. The frequencies of kdr mutation (CTT to TTT) of SeVGSC of field populations ranged China were from 60% to 89.6%. The CTT to CAT substitution at this coding position resulting in the L1014H (kdr-H) mutation was found in only one individual from the QP18 population. Based on the phylogeny of SeVGSC alleles, it appeared that the kdr mutation in S. exigua populations had multiple origins, which has major consequences for pyrethroid effectiveness in the field. Thus, it is recommended to limit the use of pyrethroid and encourage rotation of insecticides with different modes of action for control of S. exigua to alleviate resistance development.

Keywords: Spodoptera exigua; kdr; L1014F; L1014H; resistance origin.