Ultra-Sharp Nanowire Arrays Natively Permeate, Record, and Stimulate Intracellular Activity in Neuronal and Cardiac Networks

Ren Liu; Jihwan Lee; Youngbin Tchoe; Deborah Pre; Andrew M Bourhis; Agnieszka D'Antonio-Chronowska; Gaelle Robin; Sang Heon Lee; Yun Goo Ro; Ritwik Vatsyayan; Karen J Tonsfeldt; Lorraine A Hossain; M Lisa Phipps; Jinkyoung Yoo; John Nogan; Jennifer S Martinez; Kelly A Frazer; Anne G Bang; Shadi A Dayeh

doi:10.1002/adfm.202108378

Ultra-Sharp Nanowire Arrays Natively Permeate, Record, and Stimulate Intracellular Activity in Neuronal and Cardiac Networks

Adv Funct Mater. 2022 Feb 16;32(8):2108378. doi: 10.1002/adfm.202108378. Epub 2021 Nov 6.

Authors

Ren Liu¹, Jihwan Lee¹, Youngbin Tchoe¹, Deborah Pre², Andrew M Bourhis¹, Agnieszka D'Antonio-Chronowska³, Gaelle Robin², Sang Heon Lee¹, Yun Goo Ro¹, Ritwik Vatsyayan¹, Karen J Tonsfeldt⁴, Lorraine A Hossain¹, M Lisa Phipps⁵, Jinkyoung Yoo⁵, John Nogan⁶, Jennifer S Martinez⁷, Kelly A Frazer³, Anne G Bang², Shadi A Dayeh¹

Affiliations

¹ Integrated Electronics and Biointerfaces Laboratory, Department of Electrical and Computer Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
² Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
³ Department of Pediatrics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
⁴ Integrated Electronics and Biointerfaces Laboratory, Department of Electrical and Computer Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Center for Reproductive Science and Medicine, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
⁵ Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
⁶ Center for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, NM 87185, USA.
⁷ Center for Materials Interfaces in Research and Applications and Department of Applied Physics and Materials Science, Northern Arizona University, 624 S. Knoles Dr. Flagstaff, AZ 86011.

Abstract

We report innovative scalable, vertical, ultra-sharp nanowire arrays that are individually addressable to enable long-term, native recordings of intracellular potentials. Stable amplitudes of intracellular potentials from 3D tissue-like networks of neurons and cardiomyocytes are obtained. Individual electrical addressability is necessary for high-fidelity intracellular electrophysiological recordings. This study paves the way toward predictive, high-throughput, and low-cost electrophysiological drug screening platforms.

Keywords: cardiomyocyte; culture; intracellular; nanowire; neuron; tissue.

Abstract

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