hsa_circ_0001955 Promotes Colorectal Cancer Progression by Regulating miR-583/FGF21 Axis

Xuefeng Gao; Junfeng Yin; Ying Yao

doi:10.1155/2022/4288474

hsa_circ_0001955 Promotes Colorectal Cancer Progression by Regulating miR-583/FGF21 Axis

J Oncol. 2022 May 11:2022:4288474. doi: 10.1155/2022/4288474. eCollection 2022.

Authors

Xuefeng Gao¹, Junfeng Yin², Ying Yao³

Affiliations

¹ Department of Gastroenterology, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225000, China.
² Department of General Surgery, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225000, China.
³ Department of Medical Laboratory, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225000, China.

Abstract

Objective: Hsa_circ_0001955 presents significant upregulation in colorectal cancer (CRC) tissues. However, its role in CRC remains unclear. Thus, we attempted to clarify functions of hsa_circ_0001955 on CRC.

Methods: qRT-PCR was performed to examine hsa_circ_0001955, miR-583, and FGF21 levels. Western blotting was conducted to measure FGF21 protein expression. CCK-8, flow cytometry, and Ki-67 immunohistochemical staining and TUNEL assays were conducted to assess proliferation and apoptosis in vitro and in vivo, respectively. Cell invasion and migration were assessed by Transwell assay. Tumor-bearing mouse model and HE staining were used to assess inflammatory injury. Luciferase reporter system and RNA pull-down were conducted to evaluate the regulation between miR-583 and hsa_circ_0001955 or FGF21.

Results: We found that hsa_circ_0001955 showed characteristics of upregulated circRNA in CRC. Further analysis indicated that hsa_circ_0001955 elevation facilitated CRC cell malignancy in vitro and promoted tumor growth in vivo. Furthermore, hsa_circ_0001955 was a miR-583 sponge and FGF21 was directly targeted by miR-583. In addition, we found that downregulation of miR-583 promoted hsa_circ_0001955-mediated CRC cell malignancy in vitro. In contrast, FGF21 elevation promoted miR-583-regulated CRC cell malignancy in vitro.

Conclusion: We demonstrated that hsa_circ_0001955 facilitated CRC progression via miR-583/FGF21 axis, suggesting that hsa_circ_0001955 may provide a novel insight for therapy of CRC.