An Electrochemical Biosensing Platform for the SARS-CoV-2 Spike Antibody Detection Based on the Functionalised SARS-CoV-2 Spike Antigen Modified Electrode

Lokman Liv; Hilal Kayabay

doi:10.1002/slct.202200256

An Electrochemical Biosensing Platform for the SARS-CoV-2 Spike Antibody Detection Based on the Functionalised SARS-CoV-2 Spike Antigen Modified Electrode

ChemistrySelect. 2022 Mar 15;7(10):e202200256. doi: 10.1002/slct.202200256. Epub 2022 Mar 14.

Authors

Lokman Liv¹, Hilal Kayabay¹

Affiliation

¹ Electrochemistry Laboratory Chemistry Group The Scientific and Technological Research Council of Turkey National Metrology Institute TUBITAK UME) 41470 Gebze Kocaeli Turkey.

Abstract

We developed an electrochemical biosensing platform using gold-clusters, cysteamine, the spike protein of the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) antigen and bovine serum albumin on a glassy carbon electrode able to determine the SARS-CoV-2 spike antibody. The developed biosensor could detect 9.3 ag/mL of the SARS-CoV-2 spike antibody in synthetic media in 20 min in a linear range from 0.1 fg/mL to 10.0 pg/mL. The developed method demonstrated good selectivity in the presence of spike antigens from other viruses. Clinical samples consisting of gargle and mouthwash liquids were analyzed with both RT-PCR and the developed biosensor system to reveal the sensitivity and specificity of the proposed method. Moreover, the developed method was compared with the lateral flow immunoassay method in terms of sensitivity.

Keywords: Antibody determination; COVID-19; SARS-CoV-2; biosensors; gold.