Current state and future perspectives of cytochrome P450 enzymes for C-H and C=C oxygenation

Yu Yan; Jing Wu; Guipeng Hu; Cong Gao; Liang Guo; Xiulai Chen; Liming Liu; Wei Song

doi:10.1016/j.synbio.2022.04.009

Current state and future perspectives of cytochrome P450 enzymes for C-H and C=C oxygenation

Synth Syst Biotechnol. 2022 May 8;7(3):887-899. doi: 10.1016/j.synbio.2022.04.009. eCollection 2022 Sep.

Authors

Yu Yan^{1

2}, Jing Wu², Guipeng Hu², Cong Gao¹, Liang Guo¹, Xiulai Chen¹, Liming Liu¹, Wei Song²

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
² School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.

Abstract

Cytochrome P450 enzymes (CYPs) catalyze a series of C-H and C=C oxygenation reactions, including hydroxylation, epoxidation, and ketonization. They are attractive biocatalysts because of their ability to selectively introduce oxygen into inert molecules under mild conditions. This review provides a comprehensive overview of the C-H and C=C oxygenation reactions catalyzed by CYPs and the various strategies for achieving higher selectivity and enzymatic activity. Furthermore, we discuss the application of C-H and C=C oxygenation catalyzed by CYPs to obtain the desired chemicals or pharmaceutical intermediates in practical production. The rapid development of protein engineering for CYPs provides excellent biocatalysts for selective C-H and C=C oxygenation reactions, thereby promoting the development of environmentally friendly and sustainable production processes.

Keywords: Biocatalysis; C–H and C=C oxygenation; P450 enzymes.