The 5-HT and PLC Signaling Pathways Regulate the Secretion of IL-1 β, TNF- α and BDNF from NG2 Cells

Tingting Yang; Yue Li; Hui Wang; Peng Shi; Liu Teng; Haibo Guo; Xiaohua Tu; Xinyu Yao

doi:10.1155/2022/7425538

The 5-HT and PLC Signaling Pathways Regulate the Secretion of IL-1 β, TNF- α and BDNF from NG2 Cells

Evid Based Complement Alternat Med. 2022 May 13:2022:7425538. doi: 10.1155/2022/7425538. eCollection 2022.

Authors

Tingting Yang¹, Yue Li¹, Hui Wang¹, Peng Shi¹, Liu Teng¹, Haibo Guo¹, Xiaohua Tu¹, Xinyu Yao²

Affiliations

¹ School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
² Graduate School, Chengde Medical College, Chengde, China.

Abstract

The present study was clarified the relationship between NG2 glial cells and 5-hydroxytryptamine (5-HT) to further revealed a role in the regulation of cortical excitability. The co-localization of NG2 cells and 5-HT in rat prefrontal cortex was determined using immunofluorescence. Different concentrations of 5-HT were applied to cultured NG2 cells. Real-time PCR measured the expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and brain-derived neurotrophic factor (BDNF). Changes in the expression of IL-1β, TNF-α, and BDNF in NG2 cells were detected after the addition of 5-HT receptor specific blockers and phospholipase C (PLC) specific activators and inhibitors. The results confirmed that the NG2 protein and 5-HT co-localized in the prefrontal cortex. 5-HT treatment of NG2 cells significantly reduced the expression of IL-1β and BDNF mRNA and increased the expression of TNF-α. The 5-HT receptor specific inhibitors alverine citrate, ketanserin, ondansetron and SB-399885 blocked the regulatory effects of 5-HT on NG2 cells. The PLC signal was linked to the secretion of IL-1β, TNF-α and BDNF in NG2 cells. These results indicated that 5-HT affected IL-1β, TNF-α, and BDNF secretion from NG2 cells via the 5-HT1A, 5-HT2A, 5-HT3, 5-HT6 receptors and the PLC signaling pathway.