FAR1/FHY3 Transcription Factors Positively Regulate the Salt and Temperature Stress Responses in Eucalyptus grandis

Jiahao Dai; Jin Sun; Wenjing Peng; Wenhai Liao; Yuhan Zhou; Xue-Rong Zhou; Yuan Qin; Yan Cheng; Shijiang Cao

doi:10.3389/fpls.2022.883654

FAR1/FHY3 Transcription Factors Positively Regulate the Salt and Temperature Stress Responses in Eucalyptus grandis

Front Plant Sci. 2022 May 4:13:883654. doi: 10.3389/fpls.2022.883654. eCollection 2022.

Authors

Jiahao Dai^{1

2}, Jin Sun³, Wenjing Peng¹, Wenhai Liao^{1

2}, Yuhan Zhou¹, Xue-Rong Zhou⁴, Yuan Qin^{5

6

7

8}, Yan Cheng^{5

6

9}, Shijiang Cao^{1

2}

Affiliations

¹ College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China.
² University Key Laboratory of Forest Stress Physiology, Ecology and Molecular Biology of Fujian Province, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China.
³ College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China.
⁴ Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, ACT, Australia.
⁵ Fujian Agriculture and Forestry University and University of Illinois at Urbana-Champaign School of Integrative Biology Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Science, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China.
⁶ Key Laboratory of Genetics, Breeding and Multiple Utilization of Corps, College of Life Science, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China.
⁷ Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, China.
⁸ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning, China.
⁹ College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.

Abstract

FAR-RED ELONGATED HYPOCOTYLS3 (FHY3) and its homolog FAR-RED IMPAIRED RESPONSE1 (FAR1), which play pivotal roles in plant growth and development, are essential for the photo-induced phyA nuclear accumulation and subsequent photoreaction. The FAR1/FHY3 family has been systematically characterized in some plants, but not in Eucalyptus grandis. In this study, genome-wide identification of FAR1/FHY3 genes in E. grandis was performed using bioinformatic methods. The gene structures, chromosomal locations, the encoded protein characteristics, 3D models, phylogenetic relationships, and promoter cis-elements were analyzed with this gene family. A total of 33 FAR1/FHY3 genes were identified in E. grandis, which were divided into three groups based on their phylogenetic relationships. A total of 21 pairs of duplicated repeats were identified by homology analysis. Gene expression analysis showed that most FAR1/FHY3 genes were differentially expressed in a spatial-specific manner. Gene expression analysis also showed that FAR1/FHY3 genes responded to salt and temperature stresses. These results and observation will enhance our understanding of the evolution and function of the FAR1/FHY3 genes in E. grandis and facilitate further studies on the molecular mechanism of the FAR1/FHY3 gene family in growth and development regulations, especially in response to salt and temperature.

Keywords: Eucalyptus grandis; FAR1/FHY3; salt stress; temperature stress; transcription factors.