Simplified floral dip transformation method of Arabidopsis thaliana

Iftikhar Ali; Hassan Sher; Ahmad Ali; Shahid Hussain; Zahid Ullah

doi:10.1016/j.mimet.2022.106492

Simplified floral dip transformation method of Arabidopsis thaliana

J Microbiol Methods. 2022 Jun:197:106492. doi: 10.1016/j.mimet.2022.106492. Epub 2022 May 19.

Authors

Iftikhar Ali¹, Hassan Sher², Ahmad Ali², Shahid Hussain², Zahid Ullah²

Affiliations

¹ Centre for Plant Science and Biodiversity, University of Swat, Charbagh 19120, Pakistan; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: iftikhar@genetics.ac.cn.
² Centre for Plant Science and Biodiversity, University of Swat, Charbagh 19120, Pakistan.

PMID: 35597520
DOI: 10.1016/j.mimet.2022.106492

Abstract

Being a model plant, Arabidopsis transformation is a frequent routine in genetics and molecular laboratories. Here, we explain a modified floral dip method routinely used in our laboratory for Arabidopsis transformation by applying drought stress after plant treatment with Agrobacterium. By using this method, hundreds of individual transgenic plants are produced from just 30 infiltrated plants by increasing transformation frequency from 1% to 10% within 2-3 months.

Keywords: Agrobacterium tumefaciens; Arabidopsis; Drought stress; Floral dip; Transformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium / genetics
Agrobacterium tumefaciens / genetics
Arabidopsis* / genetics
Arabidopsis* / microbiology
Plants, Genetically Modified / genetics
Plants, Genetically Modified / microbiology
Transformation, Genetic