CRISPR-Cas-mediated diagnostics

Luoxi Li; Guanwang Shen; Min Wu; Jianxin Jiang; Qingyou Xia; Ping Lin

doi:10.1016/j.tibtech.2022.04.006

CRISPR-Cas-mediated diagnostics

Trends Biotechnol. 2022 Nov;40(11):1326-1345. doi: 10.1016/j.tibtech.2022.04.006. Epub 2022 May 17.

Authors

Luoxi Li¹, Guanwang Shen², Min Wu³, Jianxin Jiang⁴, Qingyou Xia⁵, Ping Lin⁶

Affiliations

¹ Biological Science Research Center, Southwest University, Chongqing 400716, China; Wound Trauma Medical Center, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China.
² Biological Science Research Center, Southwest University, Chongqing 400716, China; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
³ Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58203, USA. Electronic address: min.wu@und.edu.
⁴ Wound Trauma Medical Center, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China. Electronic address: hellojjx@126.com.
⁵ Biological Science Research Center, Southwest University, Chongqing 400716, China; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. Electronic address: xiaqy@swu.edu.cn.
⁶ Biological Science Research Center, Southwest University, Chongqing 400716, China; Wound Trauma Medical Center, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. Electronic address: linpingswu@swu.edu.cn.

PMID: 35595574
DOI: 10.1016/j.tibtech.2022.04.006

Abstract

An ideal molecular diagnostic method should be sensitive, specific, low cost, rapid, portable, and easy to operate. Traditional nucleic acid detection methods based mainly on PCR technology have not only high sensitivity and specificity, but also some limitations, such as the need for expensive equipment and skilled technicians, being both time and labor intensive, and difficult to implement in some regions. However, with the continuous development of CRISPR-Cas technology and its application in molecular diagnosis, new approaches have been used for the construction of molecular diagnostic systems. In this review, we discuss recent advances in CRISPR-based molecular diagnostic technologies and highlight the revolution they bring to the field of molecular diagnostics.

Keywords: CRISPR-Cas system; CRISPR-Cas toolbox; disease detection; molecular diagnosis; optimization application strategies.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems
Gene Editing* / methods
Molecular Diagnostic Techniques / methods
Nucleic Acids*

Substances

Nucleic Acids