Passion fruit seed extract protects beta-amyloid-induced neuronal cell death in a differentiated human neuroblastoma SH-SY5Y cell model

Akira Sato; Nozomi Tagai; Yoko Ogino; Haruka Uozumi; Shinpei Kawakami; Takayuki Yamamoto; Sei-Ichi Tanuma; Hiroko Maruki-Uchida; Sadao Mori; Minoru Morita

doi:10.1002/fsn3.2757

Passion fruit seed extract protects beta-amyloid-induced neuronal cell death in a differentiated human neuroblastoma SH-SY5Y cell model

Food Sci Nutr. 2022 Feb 2;10(5):1461-1468. doi: 10.1002/fsn3.2757. eCollection 2022 May.

Authors

Akira Sato^{1

2}, Nozomi Tagai^{1

2}, Yoko Ogino^{1

2

3}, Haruka Uozumi¹, Shinpei Kawakami⁴, Takayuki Yamamoto⁴, Sei-Ichi Tanuma^{2

5}, Hiroko Maruki-Uchida⁴, Sadao Mori⁴, Minoru Morita⁴

Affiliations

¹ Department of Biochemistry and Molecular Biology Faculty of Pharmaceutical Sciences Tokyo University of Science Noda, Chiba Japan.
² Department of Biochemistry Faculty of Pharmaceutical Sciences Tokyo University of Science Noda, Chiba Japan.
³ Present address: Department of Gene Regulation Faculty of Pharmaceutical Sciences Tokyo University of Science Noda, Chiba Japan.
⁴ Research and Development Institute Health Science Research Center, Morinaga & Co., Ltd. Yokohama Japan.
⁵ Department of Genomic Medicinal Science Research Institute for Science and Technology Organization for Research Advancement Tokyo University of Science Noda, Chiba Japan.

Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative disease with accompanying perceptive disorder. We previously reported that decreasing levels of brain-derived neurotrophic factor (BDNF) promoted beta-amyloid (Aβ)-induced neuronal cell death in neuron-like differentiated SH-SY5Y (ndSH-SY5Y) human neuroblastoma cells in an AD mimic cell model. We investigated the neuroprotective effects of passion fruit seed extract (PFSE) and one of the main stilbene compounds, piceatannol, in an AD cell model using ndSH-SY5Y cells. Both PFSE and piceatannol were found to protect Aβ-induced neurite fragmentation in the cell model (protection efficacy; 34% in PFSE and 36% in piceatannol). In addition, both PFSE and piceatannol suppress Aβ-induced neuronal cell death in the cell model (inhibitory effect; 27% in PFSE and 32% in piceatannol). Our study is the first to report that piceatannol-rich PFSE can repress Aβ-induced neuronal cell death by protecting against neurite fragmentation in the AD human cell model. These findings suggest that piceatannol-rich PFSE can be considered a potentially neuroprotective functional food for both prevention and treatment of AD.

Keywords: Alzheimer's disease; functional food; neurite fragmentation; neuronal cell death; passion fruit seed extract; piceatannol.