Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Soichiro Sonoda; Haruyoshi Yamaza; Koichiro Yoshimaru; Tomoaki Taguchi; Takayoshi Yamaza

doi:10.1016/j.xpro.2022.101386

Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

STAR Protoc. 2022 May 13;3(2):101386. doi: 10.1016/j.xpro.2022.101386. eCollection 2022 Jun 17.

Authors

Soichiro Sonoda¹, Haruyoshi Yamaza², Koichiro Yoshimaru³, Tomoaki Taguchi^{3

4}, Takayoshi Yamaza¹

Affiliations

¹ Department of Molecular Biology and Oral Anatomy, Kyushu University Graduate School of Dental Science, Fukuoka 812-8582, Japan.
² Department of Pediatric Dentistry, Kyushu University Graduate School of Dental Science, Fukuoka 812-8582, Japan.
³ Department of Pediatric Surgery, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582, Japan.
⁴ Fukuoka College of Health Sciences, Fukuoka 814-0193, Japan.

Abstract

Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).

Keywords: Cell isolation; Clinical Protocol; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Dental Pulp*
Humans
Regeneration*
Stem Cell Transplantation