Evaluation of NF1 and RASA1 gene expression in endometriosis

Ghafour Yarahmadi; Mehran Dehghanian; Reyhaneh Sadat Sandoghsaz; Mohamadreza Savaee; Farimah Shamsi; Mohammad Yahya Vahidi Mehrjardi

doi:10.1016/j.eurox.2022.100152

Evaluation of NF1 and RASA1 gene expression in endometriosis

Eur J Obstet Gynecol Reprod Biol X. 2022 May 4:15:100152. doi: 10.1016/j.eurox.2022.100152. eCollection 2022 Aug.

Authors

Ghafour Yarahmadi^{1

2}, Mehran Dehghanian^{1

2}, Reyhaneh Sadat Sandoghsaz², Mohamadreza Savaee³, Farimah Shamsi², Mohammad Yahya Vahidi Mehrjardi^{2

4}

Affiliations

¹ Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
² Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
³ Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
⁴ Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abstract

Background: Endometrios affecting 6-10% of women of reproductive ages around the globe. Important pathways, including the MAPK and PI3K / Akt pathways, have been identified in the disease. The NF1 and RASA1 genes inactivate Ras by their own GTPase activity and controlled the high activity of these pathways.

Objective: In this study, we measured NF1 and RASA1 gene expression in the endometrial tissues of patients (eutopic and ectopic tissues) compared to the control samples.

Materials and methods: In our study, tissue samples were collected from 15 patients with endometriosis and 15 healthy women. We used quantitative polymerase chain reaction (qRT-PCR) to measure the NF1 and RASA1 gene expression levels in these samples.

Results: We observed a significant decrease in the expression level of the NF1 gene in both eutopic and ectopic samples of endometriosis patients compared to control samples, while the expression of the RASA1 gene was significantly reduced only in ectopic tissues.

Keywords: Endometriosis; MAPK; NF1; PI3K / Akt; RASA1.