Application of Immunoaffinity Mass Spectrometry (IA-MS) for Protein Biomarker Quantification

Joe Palandra; Nikolaos Psychogios; Hendrik Neubert

doi:10.1007/978-1-0716-2176-9_8

Application of Immunoaffinity Mass Spectrometry (IA-MS) for Protein Biomarker Quantification

Methods Mol Biol. 2022:2466:111-119. doi: 10.1007/978-1-0716-2176-9_8.

Authors

Joe Palandra¹, Nikolaos Psychogios², Hendrik Neubert²

Affiliations

¹ Pfizer Worldwide Research Development and Medical, Andover, MA, USA. joe.palandra@pfizer.com.
² Pfizer Worldwide Research Development and Medical, Andover, MA, USA.

PMID: 35585314
DOI: 10.1007/978-1-0716-2176-9_8

Abstract

Immunoaffinity mass spectrometry (IA-MS) is a powerful analytical technique for the determination of protein biomarkers with high sensitivity and unparalleled specificity. Typically, the protein antigen of interest is captured from biofluids and tissue lysates using an antibody prior to mass spectrometric analysis. Here we describe the specific steps of the protein immunoaffinity component of the IA-MS workflow that is applicable to most protein antigens.

Keywords: Biomarker quantification; Immunoaffinity mass spectrometry (IA-MS ).

MeSH terms

Antibodies* / chemistry
Antigens
Biomarkers / analysis
Mass Spectrometry / methods
Proteins* / analysis

Substances

Antibodies
Antigens
Biomarkers
Proteins