Tropical forest serves as an important pivotal role in terrestrial biological diversity. The present study makes an attempt to identify the concentration of species among tree diversity in Mahavir Swami Wildlife Sanctuary, Bundelkhand, India. Four important ecological indicator indices namely Shannon-Weiner index (H'), Simpson's diversity (D), Margalef index (SR) and Pielou's (J) indices were make the most for species diversity measurement. The research outcomes revealed that Shannon-Weiner diversity index (H/) was found to be the best index for assessing species richness while Simpson's diversity (D) index was more suited for determining species diversity. The Shannon-Weiner index value calculated for different transects not only represent the species richness but also the species evenness in each transect. The potential application of forest diversity can be used a mechanism for forest management. The methodology will retrofit better policy implementation for maintaining the health of forest species in Mahavir Swami Wildlife Sanctuary and can be applied on other reserve forest of socio-ecological significance.