Ratiometric imaging of butyrylcholinesterase activity in mice with nonalcoholic fatty liver using an AIE-based fluorescent probe

Chunbai Xiang; Jingjing Xiang; Xing Yang; Chunbin Li; Lihua Zhou; Daoyong Jiang; Yonglin Peng; Zhen Xu; Guanjun Deng; Baode Zhu; Pengfei Zhang; Lintao Cai; Ping Gong

doi:10.1039/d2tb00422d

Ratiometric imaging of butyrylcholinesterase activity in mice with nonalcoholic fatty liver using an AIE-based fluorescent probe

J Mater Chem B. 2022 Jun 8;10(22):4254-4260. doi: 10.1039/d2tb00422d.

Authors

Chunbai Xiang^{1

2}, Jingjing Xiang¹, Xing Yang^{1

2}, Chunbin Li³, Lihua Zhou⁴, Daoyong Jiang¹, Yonglin Peng⁵, Zhen Xu¹, Guanjun Deng¹, Baode Zhu⁶, Pengfei Zhang¹, Lintao Cai¹, Ping Gong¹

Affiliations

¹ Guangdong Key Laboratory of Nanomedicine, CAS Key Laboratory of Health Informatics, Shenzhen Bioactive Materials Engineering Lab for Medicine, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. pf.zhang@siat.ac.cn.
² University of Chinese Academy of Sciences, Beijing 100049, China.
³ College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, P. R. China.
⁴ School of Applied Biology, Shenzhen Institute of Technology, No. 1 Jiangjunmao, Shenzhen 518116, P. R. China.
⁵ Pinete (Zhongshan) Biotechnology Co., Ltd. Digital trade building, No. 6, Xiangxing Road, Torch Development Zone, Zhongshan, 528400, China.
⁶ School of Chemistry and Environmental Science, Xiangnan University, Chenzhou 423000, China.

PMID: 35583194
DOI: 10.1039/d2tb00422d

Abstract

Butyrylcholinesterase (BChE) is an essential human biomarker which is related to liver and neurodegenerative diseases. It is of great significance to develop a fluorescent probe that can image BChE in vitro and in vivo. Unfortunately, most fluorescent probes that are based on a single change in fluorescence intensity are susceptible to environmental interference. Therefore, we reported an easily available ratiometric fluorescent probe, TB-BChE, with aggregation-induced emission (AIE) characteristics for ratiometric imaging of BChE. TB-BChE demonstrated excellent sensitivity (LOD = 39.24 ng mL^-1) and specificity for BChE. Moreover, we have successfully studied the ratiometric imaging of TB-BChE to BChE in a nonalcoholic fatty liver disease model. These results indicated that TB-BChE is expected to become a powerful analysis tool for butyrylcholinesterase research in basic medicine and clinical applications.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Butyrylcholinesterase*
Diagnostic Imaging
Fluorescent Dyes
Mice
Non-alcoholic Fatty Liver Disease* / diagnostic imaging

Substances

Fluorescent Dyes
Butyrylcholinesterase