Nanodiamonds with Surface Grafted Polymer Chains as Vehicles for Cell Imaging and Cisplatin Delivery: Enhancement of Cell Toxicity by POEGMEMA Coating

Vien T Huynh; Samuel Pearson; Janina-Miriam Noy; Amanda Abboud; Robert H Utama; Hongxu Lu; Martina H Stenzel

doi:10.1021/mz4000199

Nanodiamonds with Surface Grafted Polymer Chains as Vehicles for Cell Imaging and Cisplatin Delivery: Enhancement of Cell Toxicity by POEGMEMA Coating

ACS Macro Lett. 2013 Mar 19;2(3):246-250. doi: 10.1021/mz4000199. Epub 2013 Feb 27.

Authors

Vien T Huynh¹, Samuel Pearson¹, Janina-Miriam Noy¹, Amanda Abboud¹, Robert H Utama¹, Hongxu Lu¹, Martina H Stenzel¹

Affiliation

¹ Centre for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney NSW 2052, Australia.

PMID: 35581890
DOI: 10.1021/mz4000199

Abstract

Nanodiamonds (NDs) are highly promising drug carriers due to their biocompatibility, manipulable surface chemistry, and nonbleaching flourescence. In this communication, we compare the cytotoxicity of three ND-cisplatin systems in which cisplatin was incorporated via direct attachment to the ND surface, physical adsorption within a poly(oligo(ethylene glycol) methyl ether methacrylate) POEGMEMA surface coating, or complexation to 1,1-di-tert-butyl 3-(2-methacryloyloxy)ethyl)butane-1,1,3-tricarboxylate (MAETC) groups of a POEGMEMA-st-PMAETC surface layer. The polymer layers were introduced by grafting from RAFT-functionalized ND particles. All three ND systems displayed lower IC₅₀ values than free cisplatin in A2870 and A2870cis ovarian cancer cells. The two polymer-containing systems outperformed their "naked" counterpart, with the POEGMEMA-coated particles the most cytotoxic, displaying an IC₅₀ of 1.5 μM, more than an order of magnitude lower than that of cisplatin. The enhanced cytotoxicity is attributed to promotion of cellular uptake by the hydrophilic surface polymer.