Characterization of RASA-1, a novel class A extended-spectrum beta-lactamase from Riemerella anatipestifer

Hongyan Luo; Mengru Li; Zhishuang Yang; Dekang Zhu; Pei Li

doi:10.1016/j.vetmic.2022.109456

Characterization of RASA-1, a novel class A extended-spectrum beta-lactamase from Riemerella anatipestifer

Vet Microbiol. 2022 Jul:270:109456. doi: 10.1016/j.vetmic.2022.109456. Epub 2022 May 12.

Authors

Hongyan Luo¹, Mengru Li¹, Zhishuang Yang², Dekang Zhu³, Pei Li⁴

Affiliations

¹ College of Veterinary Medicine, Southwest University, Beibei, Chongqing, China.
² Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
³ Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China. Electronic address: zdk24@sicau.edu.cn.
⁴ College of Veterinary Medicine, Southwest University, Beibei, Chongqing, China. Electronic address: lipei98989@swu.edu.cn.

PMID: 35580446
DOI: 10.1016/j.vetmic.2022.109456

Abstract

A novel chromosomally-located β-lactamase gene, bla_RASA-1, was identified in Riemerella anatipestifer RA-CH-1. The RASA-1, encoded by bla_RASA-1, was a class A extended-spectrum β-lactamase (ESBL), which shared 42.7% and 40.5% identities with the RAA-1 and CGA-1 β-lactamase, respectively. Overexpression of RASA-1 in Escherichia coli confers broad resistance to β-lactams and the purified native RASA-1 revealed ESBL-like hydrolysis activity. Blasting in GenBank showed that bla_RASA-1 was exclusively detected in Riemerella anatipestifer. Moreover, sequence analysis revealed that this gene was located within the multi-resistance region of Riemerella anatipestifer genome.

Keywords: Novel β-lactamase; RASA-1; Riemerella anatipestifer.

MeSH terms

Animals
Escherichia coli / genetics
Riemerella* / enzymology
Riemerella* / genetics
beta-Lactamases* / genetics

Substances

beta-Lactamases

Supplementary concepts

Riemerella anatipestifer