Deep insights into the anaerobic co-digestion of waste activated sludge with concentrated leachate under different salinity stresses

Abstract

Treatment of high-salinity organic wastewater (e.g., concentrated leachate) is a major challenge. Anaerobic co-digestion can effectively treat high-salinity organic wastewater and recover energy. In this study, the concentrated landfill leachate and waste activated sludge (WAS) were anaerobic co-digested in the lab-scale continuous stirred tank reactors (CSTR) to understand their co-digestion performance under different salinity stresses. As revealed by the results, when the salinity was low (<10 g/L), the removal ratio of organic matter in the digester was kept at a high level (>91.3%), and the concentration of total volatile fatty acids (TVFAs) was low (<100 mg COD/L), indicating that the digester could operate efficiently and stably. However, when the salinity level was elevated from 10 g/L to 30 g/L, the removal ratio of organic matter in the digester decreased from ~91.3% to ~64.5%, the TVFAs continued to accumulate, the yields of biogas and methane also dropped sharply, and the performance of the digester decreased gradually. The results of microbial community and diversity analysis showed that there is limited adaptability of microbial community to high salinity in such process. Salinity could cause significant changes in the microbial community and diversity, thereby affecting the digestive performance. Metagenomic analysis showed that under high salinity conditions, the content of genes encoding hydrolase and methanogenic enzyme decreased, whereas the pathway of acetotrophic methanogenesis was weakened. Mechanism study showed that with the increase of salinity, the activity of microbial cells decreased, the structure of sludge flocs was damaged more significantly, and the extracellular polymeric substances (EPS) secreted by microbe increased continuously, which was used to resist the toxic effects of salinity stresses on microorganisms. The results of this study could provide certain theoretical guidance for anaerobic digestion under salinity stresses.

Keywords: Anaerobic co-digestion; Concentrated leachate; Metabolic pathway; Salinity; Waste activated sludge.