African Swine Fever Virus Plaque Assay and Disinfectant Testing

Lorraine Frost; Carrie Batten

doi:10.1007/978-1-0716-2333-6_14

African Swine Fever Virus Plaque Assay and Disinfectant Testing

Methods Mol Biol. 2022:2503:187-194. doi: 10.1007/978-1-0716-2333-6_14.

Authors

Lorraine Frost¹, Carrie Batten²

Affiliations

¹ Non-vesicular Reference Laboratory, The Pirbright Institute, Pirbright, Woking, UK.
² Non-vesicular Reference Laboratory, The Pirbright Institute, Pirbright, Woking, UK. carrie.batten@pirbright.ac.uk.

PMID: 35575896
DOI: 10.1007/978-1-0716-2333-6_14

Abstract

Quantifying the titer of African swine fever virus is critical for disease control, viral infection studies, and disinfectant efficacy tests. Techniques such as real-time PCR and virus isolation provide an understanding as to whether viral genome is present or gives a qualitative assessment of live viral presence in a sample respectively, but neither provide a quantitative measurement of live virus. Here we describe a plaque assay for the titration of a Vero-adapted African swine fever virus strain (BA71V) and describe how to apply this method to determine disinfectant efficacy.

Keywords: African swine fever virus; Disinfectant; Plaque assay.

MeSH terms

African Swine Fever Virus* / genetics
African Swine Fever* / genetics
Animals
DNA Viruses / genetics
Disinfectants*
Genome, Viral
Real-Time Polymerase Chain Reaction
Swine

Substances

Disinfectants