African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- purified Antigen (ASFV CP-Ag)

Gallardo Carmina; R Nieto; M Arias

doi:10.1007/978-1-0716-2333-6_9

African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- purified Antigen (ASFV CP-Ag)

Methods Mol Biol. 2022:2503:133-145. doi: 10.1007/978-1-0716-2333-6_9.

Authors

Gallardo Carmina¹, R Nieto², M Arias²

Affiliations

¹ European Union Reference Laboratory for African Swine Fever (EURL), Centro de Investigación en Sanidad Animal, CISA-INIA/CSIC, Valdeolmos, Madrid, Spain. gallardo@inia.es.
² European Union Reference Laboratory for African Swine Fever (EURL), Centro de Investigación en Sanidad Animal, CISA-INIA/CSIC, Valdeolmos, Madrid, Spain.

PMID: 35575891
DOI: 10.1007/978-1-0716-2333-6_9

Abstract

The present chapter describes a simple and economic indirect enzyme immunoassay (ELISA ) for African swine fever virus (ASFV) antibody detection based on the use of the soluble cytoplasmic fraction of ASFV-infected monkey stable cells (MS). The soluble antigen proteins of ASFV-infected cells are separated by sucrose precipitation centrifugation, and the supernatant above the sucrose layer is used as an ELISA antigen. The test serum sample reacts with the cytoplasmic soluble fraction, and antibodies are detected using a protein A-peroxidase conjugate. This crude antigen is currently recommended as a test reagent in screening and diagnostic tests by the World Organization for Animal Health (OIE).

Keywords: ASFV antibodies; African swine fever virus (ASFV); ELISA; Soluble cytoplasmic antigen.

MeSH terms

African Swine Fever Virus*
African Swine Fever* / diagnosis
Animals
Antibodies, Viral
Enzyme-Linked Immunosorbent Assay
Sucrose
Swine

Substances

Antibodies, Viral
Sucrose