Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction

Christopher L Netherton; Lynnette C Goatley; John Flannery; Martin Ashby; Carrie Batten

doi:10.1007/978-1-0716-2333-6_6

Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction

Methods Mol Biol. 2022:2503:95-104. doi: 10.1007/978-1-0716-2333-6_6.

Authors

Christopher L Netherton¹, Lynnette C Goatley¹, John Flannery², Martin Ashby², Carrie Batten³

Affiliations

¹ African Swine Fever Vaccinology Group, The Pirbright Institute, Pirbright, Woking, UK.
² Non-vesicular Reference Laboratory, The Pirbright Institute, Pirbright, Woking, UK.
³ Non-vesicular Reference Laboratory, The Pirbright Institute, Pirbright, Woking, UK. carrie.batten@pirbright.ac.uk.

PMID: 35575888
DOI: 10.1007/978-1-0716-2333-6_6

Abstract

Real-time polymerase chain reaction (PCR) for the detection of African swine fever virus (ASFV) is the tool of choice for the diagnostic laboratory and is a robust and easily scalable method for the researcher analyzing viral replication both in vitro and in vivo. In this chapter, we describe protocols for both quantitative real-time polymerase chain reactions (qPCR) and non-quantitative real-time polymerase chain reactions (real-time PCR) for the detection of African swine fever virus genome in a range of samples.

Keywords: African swine fever; Diagnosis; Real-time PCR; TaqMan®; qPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

African Swine Fever Virus* / genetics
African Swine Fever* / diagnosis
Animals
Clinical Laboratory Techniques
Real-Time Polymerase Chain Reaction / methods
Sensitivity and Specificity
Swine

Abstract

Publication types

MeSH terms

Grants and funding