Understanding Willow Transcriptional Response in the Context of Oil Sands Tailings Reclamation

Abdul Samad; Gervais Pelletier; Armand Séguin; Dani Degenhardt; Douglas G Muench; Christine Martineau

doi:10.3389/fpls.2022.857535

Understanding Willow Transcriptional Response in the Context of Oil Sands Tailings Reclamation

Front Plant Sci. 2022 Apr 27:13:857535. doi: 10.3389/fpls.2022.857535. eCollection 2022.

Authors

Abdul Samad¹, Gervais Pelletier¹, Armand Séguin¹, Dani Degenhardt², Douglas G Muench³, Christine Martineau¹

Affiliations

¹ Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Québec City, QC, Canada.
² Natural Resources Canada, Canadian Forest Service, Northern Forestry Centre, Edmonton, AB, Canada.
³ Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.

Abstract

One of the reclamation objectives for treated oil sands tailings (OST) is to establish boreal forest communities that can integrate with the surrounding area. Hence, selection of appropriate soil reclamation cover designs and plant species for revegetation are important aspects of tailings landform reclamation and closure. Research and monitoring of the long term and immediate impacts of capped OST on the growth and survival of native boreal plant species are currently underway. However, plant responses to OST-associated toxicity are not well known at the molecular level. Using RNA sequencing, we examined the effects of three types of OST on the willow transcriptome under different capping strategies. The transcriptomic data showed that some genes respond universally and others in a specific manner to different types of OST. Among the dominant and shared upregulated genes, we found some encoding protein detoxification (PD), Cytochrome P450 (CYPs), glutathione S-transferase regulatory process (GST), UDP-glycosyltransferase (UGT), and ABC transporter and regulatory process associated proteins. Moreover, genes encoding several stress-responsive transcription factors (bZIP, BHLH, ERF, MYB, NAC, WRKY) were upregulated with OST-exposure, while high numbers of transcripts related to photosynthetic activity and chloroplast structure and function were downregulated. Overall, the expression of 40 genes was found consistent across all tailings types and capping strategies. The qPCR analysis of a subset of these shared genes suggested that they could reliably distinguish plants exposed to different OST associated stress. Our results indicated that it is possible to develop OST stress exposure biosensors merely based on changes in the level of expression of a relatively small set of genes. The outcomes of this study will further guide optimization of OST capping and revegetation technology by using knowledge based plant stress adaptation strategies.

Keywords: oil sands tailings; polycyclic aromatic hydrocarbon; reclamation; transcriptome; willow.