Fabricating self-powered microfluidic devices via 3D printing for manipulating fluid flow

Sung Oh Woo; Myungkeun Oh; Yongki Choi

doi:10.1016/j.xpro.2022.101376

Fabricating self-powered microfluidic devices via 3D printing for manipulating fluid flow

STAR Protoc. 2022 May 7;3(2):101376. doi: 10.1016/j.xpro.2022.101376. eCollection 2022 Jun 17.

Authors

Sung Oh Woo¹, Myungkeun Oh², Yongki Choi^{1

2

3}

Affiliations

¹ Department of Physics, North Dakota State University, Fargo, ND 58108, USA.
² Materials and Nanotechnology Program, North Dakota State University, Fargo, ND 58108, USA.
³ Cellular and Molecular Biology Program, North Dakota State University, Fargo, ND 58108, USA.

Abstract

Advances in 3D printing technologies allow fabrication of complex structures at micron resolution. Here, we describe two approaches of fabricating self-powered microfluidic devices utilizing 3D printing: PDMS (polydimethylsiloxane)-based microfluidic devices with a built-in vacuum pocket fabricated by soft lithography using a 3D-printed mold, and non-PDMS microfluidic devices operating by a removable vacuum battery fabricated by 3D-printed materials. These microfluidic devices can be used for controlling blood flow and separating blood plasma. For complete details on the use and execution of this protocol, please refer to Woo et al. (2021).

Keywords: Biophysics; Biotechnology and bioengineering.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Lab-On-A-Chip Devices*
Physical Phenomena
Printing / methods
Printing, Three-Dimensional*

Grants and funding

P20 GM109024/GM/NIGMS NIH HHS/United States