An SI-traceable reference material for virus-like particles

Andrea Briones; Gustavo Martos; Magali Bedu; Tiphaine Choteau; Ralf D Josephs; Robert I Wielgosz; Maxim G Ryadnov

doi:10.1016/j.isci.2022.104294

An SI-traceable reference material for virus-like particles

iScience. 2022 Apr 25;25(5):104294. doi: 10.1016/j.isci.2022.104294. eCollection 2022 May 20.

Authors

Andrea Briones^{1

2}, Gustavo Martos², Magali Bedu², Tiphaine Choteau², Ralf D Josephs², Robert I Wielgosz², Maxim G Ryadnov^{1

3}

Affiliations

¹ National Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK.
² Bureau International des Poids et Mesures, Pavillon de Breteuil, 92312 Sèvres, France.
³ Department of Physics, King's College London, London WC2R 2LS, UK.

Abstract

A reference material for virus-like particles traceable to the International System of Units (Système International d'Unités - the SI) is reported. The material addresses the need for developing reference standards to benchmark virus-like gene delivery systems and help harmonize measurement approaches for characterization and testing. The material is a major component of synthetic polypeptide virus-like particles produced by the state-of-the-art synthetic and analytical chemistry methods used to generate gene delivery systems. The purity profile of the material is evaluated to the highest metrological order demonstrating traceability to the SI. The material adds to the emerging toolkit of reference standards for quantitative biology.

Keywords: Analytical chemistry; Bioengineering; Biomaterials; Materials science; Nanotechnology.