Evaluation of IL-3, IL-5, and IL-6 concentration in the follicular fluid of women with endometriosis: A cross-sectional study

Mohammad Ghodsi; Vida Hojati; Armin Attaranzadeh; Bita Saifi

doi:10.18502/ijrm.v20i3.10713

Evaluation of IL-3, IL-5, and IL-6 concentration in the follicular fluid of women with endometriosis: A cross-sectional study

Int J Reprod Biomed. 2022 Apr 21;20(3):213-220. doi: 10.18502/ijrm.v20i3.10713. eCollection 2021 Mar.

Authors

Mohammad Ghodsi¹, Vida Hojati¹, Armin Attaranzadeh², Bita Saifi³

Affiliations

¹ Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
² Imam Reza Hospital, Mashhad University of Medical Science, Mashhad, Iran.
³ Department of Anatomy, Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran.

Abstract

Background: Endometriosis is associated with abnormal immunologic responses and combined inflammatory and anti-inflammatory conditions.

Objective: This study aims to investigate follicular fluid (FF) concentration of interleukin (IL)-3, IL-5, and IL-6 in women with and without endometriosis.

Materials and methods: In this cross-sectionalstudy 68 women who were referred to the in vitro fertilization center of Imam Reza hospital in Mashhad during 2018 were selected randomly. Leaves of cytokines in the FF samples were evaluated in the endometriosis and the control group (n = 34/each). The diagnostic accuracy of cytokines and clinical characteristics were evaluated.

Results: IL-3 and IL-6 were significantly changed in the FF of the women with endometriosis compared with the control group (p = 0.04, and p $<$ 0.01, respectively), and the mean concentration of IL-5 in the endometriosis group was lower than in the control group (p = 0.5), but this was not significant. There were significant differences in the menstrual cycle, dyspareunia, and dysmenorrhea between the groups (p $<$ 0.01, p = 0.04, and p = 0.02, respectively). The diagnostic accuracy of IL-3 and IL-6 in the FF was low, with the area under the curve of 0.614 and 0.645, respectively.

Conclusion: Although none of the cytokines had a predictive value for endometriosis, the decreased levels of IL-3 and increased levels of IL-6 in the FF samples of women with endometriosis, and risk factors, including irregular menstrual cycle, dyspareunia, and dysmenorrhea, could be associated with the pathogenesis of this painful disease.

Keywords: Endometriosis.; Follicular fluid; Interleukin-5; Interleukin-6; Interleukin-3.