Transcriptome analysis uncovering regulatory networks and hub genes of Populus photosynthesis and chlorophyll content

Kang Du; Wenqi Wu; Ting Liao; Jun Yang; Xiangyang Kang

doi:10.1016/j.ygeno.2022.110385

Transcriptome analysis uncovering regulatory networks and hub genes of Populus photosynthesis and chlorophyll content

Genomics. 2022 Jul;114(4):110385. doi: 10.1016/j.ygeno.2022.110385. Epub 2022 May 13.

Authors

Kang Du¹, Wenqi Wu¹, Ting Liao², Jun Yang¹, Xiangyang Kang³

Affiliations

¹ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
² National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Haidian District, Beijing 100083, China.
³ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: kangxy@bjfu.edu.cn.

PMID: 35569730
DOI: 10.1016/j.ygeno.2022.110385

Abstract

Photosynthesis plays vital role in plant growth and development throughout its life, and it is influenced by environmental signals and circadian rhythms. We analyzed the transcriptome landscape of the two poplars progeny with contrasting photosynthesis rates at three times point (ZT4, ZT16, ZT22), constructed gene regulatory network that related to circadian rhythm and photosynthesis. We suggest that the differences in photosynthetic rate between the progenies may originate from plant endogenous circadian oscillators prepare poplar plants for photosynthesis by regulating photosynthesis-associated nuclear genes and carotenoid metabolism genes before dawn, genes associated with plant hormone signal transduction and transcription factor increase leaf size and stomatal movement, the influence of other core regulatory factors on chlorophyll accumulation. Furthermore, overexpression of candidate regulatory gene, AP3 (Potri.007G017000), induced leaf senescence and reduced the content of chlorophyll. These results demonstrated that many potential key regulators are integrated closely with chlorophyll content and photosynthesis.

Keywords: Circadian rhythms; Leaf development; Photosynthesis; Poplar; Transcriptomic analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorophyll / metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Photosynthesis / genetics
Plant Leaves / genetics
Plant Leaves / metabolism
Populus*
Transcriptome

Substances

Chlorophyll