A novel self-aggregated gold nanoparticles based on sensitive immunochromatographic assays for highly detection of opium poppy in herbal teas

Xuan Zhang; Jin Wang; Jianhao Liang; Zhiwei Liu; Xing Shen; Yingju Liu; Xiangmei Li; Zhenlin Xu; Yi Lei; Hongtao Lei

doi:10.1016/j.foodchem.2022.133188

A novel self-aggregated gold nanoparticles based on sensitive immunochromatographic assays for highly detection of opium poppy in herbal teas

Food Chem. 2022 Oct 1:390:133188. doi: 10.1016/j.foodchem.2022.133188. Epub 2022 May 10.

Authors

Xuan Zhang¹, Jin Wang¹, Jianhao Liang¹, Zhiwei Liu¹, Xing Shen¹, Yingju Liu², Xiangmei Li¹, Zhenlin Xu¹, Yi Lei³, Hongtao Lei⁴

Affiliations

¹ Guangdong Provincial Key Laboratory of Food Quality and Safety / National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou 510642, China.
² Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
³ Guangdong Institute of Food Inspection, Guangzhou 510435, China.
⁴ Guangdong Provincial Key Laboratory of Food Quality and Safety / National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou 510642, China. Electronic address: hongtao@scau.edu.cn.

PMID: 35567969
DOI: 10.1016/j.foodchem.2022.133188

Abstract

Opium poppy abused in food has aroused public concerns due to its serious side effects. Effective monitoring is essential to fight the abuse crisis. Herein, we synthesized an easily prepared, affordable, accessible highly aggregated gold nanoparticles (AGNPs) performing in lateral flow immunoassay (LFIA) for detection opium poppy in herbal teas. Simultaneously, a LFIA based ontime-resolved fluorescent microspheres (TRFMs) was developed as contrastive method. In this study, morphine (MOR), codeine (COD) and thebaine (THE) were as the specific recognition markers of opium poppy. Results demonstrated the quantitative limits of detection were 0.0049/0.0053/0.084, 0.034/0.037/0.37 ng mL^-1 for AGNPs/TRFMs-LFIA, respectively. The recoveries were 95%-107.5%/91%-106.7% with coefficient of variation was 1.6%-6.6%/1.8%-7.2%, indicating excellent accuracy and precision. Parallel experiments among AGNPs/TRFMs-LFIA and LC-MS/MS analysis showed good correlation. Overall, AGNPs-LFIA executed quantitative analysis within 15 min on the basis of simple treatment while providing a rapid and sensitive analysis strategy for illegal drugs abused.

Keywords: Codeine; Herbal teas; Morphine; Opium poppy; Self-aggregated gold nanoparticles; Thebaine.

MeSH terms

Chromatography, Liquid
Gold
Immunoassay
Metal Nanoparticles*
Papaver* / chemistry
Tandem Mass Spectrometry
Teas, Herbal*

Substances

Teas, Herbal
Gold