Metabolomic analysis of endometrial cancer by high-resolution magic angle spinning NMR spectroscopy

Abstract

Purpose: To analyze endometrial metabolite profiles between patients with endometrial cancer and controls.

Methods: Seventeen (17) women with endometrium cancer and 18 controls were enrolled in this study. ¹H HR-MAS (High Resolution-Magic Angle Spinning) NMR (Nuclear Magnetic Resonance) spectroscopy data obtained from endometrial tissue samples of patients with endometrial cancer and control group were analyzed with bioinformatics methods.

Results: Principal component analysis (PCA) and the partial least squares discriminant analysis (PLS-DA) score plots obtained with the multivariate statistical analysis of pre-processed spectral data shows a separation between the samples from patients with endometrial cancer and controls. Analysis results suggest that the levels of lactate, glucose, o-phosphoethanolamine, choline, glycerophosphocholine, phosphocholine, leucine, isoleucine, valine, glutamate, glutamine, n-acetyltyrosine, methionine, taurine, alanine, aspartate and phenylalanine are increased in patients with endometrial cancer compared to the controls.

Conclusion: The metabolomics signature of patients with endometrial cancer is different from that of benign endometrial tissue.

Keywords: 1H-NMR; Endometrial cancer; Endometrial tissue; Metabolomics.