Extraction, Biochemical Characterization, and Health Effects of Native and Degraded Fucoidans from Sargassum crispifolium

Wei-Cheng Hsiao; Yong-Han Hong; Yung-Hsiang Tsai; Yi-Chen Lee; Anil Kumar Patel; Hui-Ru Guo; Chia-Hung Kuo; Chun-Yung Huang

doi:10.3390/polym14091812

Extraction, Biochemical Characterization, and Health Effects of Native and Degraded Fucoidans from Sargassum crispifolium

Polymers (Basel). 2022 Apr 28;14(9):1812. doi: 10.3390/polym14091812.

Authors

Wei-Cheng Hsiao¹, Yong-Han Hong², Yung-Hsiang Tsai³, Yi-Chen Lee³, Anil Kumar Patel⁴, Hui-Ru Guo³, Chia-Hung Kuo³, Chun-Yung Huang³

Affiliations

¹ Division of Gastroenterology (General Medicine), Department of Internal Medicine, Yuan's General Hospital, No. 162, Cheng Kung 1st Rd., Lingya District, Kaohsiung City 80249, Taiwan.
² Department of Nutrition, I-Shou University (Yanchao Campus), No. 8, Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung City 82445, Taiwan.
³ Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, Taiwan.
⁴ Sustainable Environment Research Center, Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, Taiwan.

Abstract

In the current investigation, a native crude fucoidan (Ex) was extracted from Sargassum crispifolium, pretreated by single-screw extrusion, and two degraded fucoidans, i.e., ExAh (degradation of Ex by ascorbic acid) and ExHp (degradation of Ex by hydrogen peroxide), were obtained. The extrusion pretreatment increased the extraction yield of fucoidan by approximately 1.73-fold as compared to the non-extruded sample. Among Ex, ExAh, and ExHp, their molecular weight and chemical compositions varied, but the structural features were similar. ExHp possessed the greatest antioxidant activities among the extracted fucoidans. According to the outcome, ExAh exhibited the maximum immune promoting effects via enhanced NO, TNF-α, IL-1β, IL-6, and IL-10 secretion. Thus, both ExHp and ExAh may potentially be used as an effective antioxidant and as immunostimulant agents, which could be of great value in the development of food and nutraceutical products.

Keywords: Fourier transform infrared; Sargassum crispifolium; antioxidant; cytokine; fucoidan; immunostimulant.

Grants and funding

This research was funded by Yuan’s General Hospital, Taiwan, grant number YUAN-IACR-22-01 to Wei-Cheng Hsiao. This work was supported by the Ministry of Science and Technology, Taiwan [grant number MOST 110-2221-E-992-011] and [grant number MOST 109-2314-B-214-004], which is awarded to Chun-Yung Huang and Yong-Han Hong, respectively. The authors thank the Ministry of Education, Taiwan, for supporting this study [grant number MOE-RSC-108RSN0005], awarded to Yong-Han Hong.