Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows

Guanxin Lv; Hai Wang; Xiechen Zhou; Shuai Lian; Jianfa Wang; Rui Wu

doi:10.3390/ani12091190

Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows

Animals (Basel). 2022 May 6;12(9):1190. doi: 10.3390/ani12091190.

Authors

Guanxin Lv^{1

2}, Hai Wang^{1

2}, Xiechen Zhou^{1

2}, Shuai Lian^{1

2}, Jianfa Wang^{1

2}, Rui Wu^{1

2}

Affiliations

¹ College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
² Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, Daqing 163319, China.

Abstract

Polymorphonuclear neutrophils (PMN) are the first line of defense against the invasion of foreign pathogenic microorganisms and play an essential role in the immune system of dairy cows. The changes in hormone secretion and metabolites of dairy cows during the perinatal period are the key factors that cause immunosuppression and increased risk of diseases. However, the effects of the hormone, nonesterified fatty acid (NEFA), and short-chain fatty acid (SCFA) on the transmammary epithelial migration of dairy cows and the formation of neutrophil extracellular traps (NETs) have rarely been studied. This study explored the effects of hormones, NEFAs and SCFAs on the neutrophil migration and NETs formation of dairy cows in vitro. It was found that P₄ and Ac can regulate the transepithelial migration of PMN; SA and Pr can regulate the formation of NETs; E₂, OA and Bt can regulate PMN transepithelial migration and NET formation. These results help to further explain the effects of changes in hormone secretion and metabolites on immunosuppression and the increased risk of disease in perinatal dairy cows.

Keywords: NEFA; NETs; SCFA; bovine; hormone; migration; neutrophils.

Grants and funding

This work was financially supported by the Natural Science Foundation of China (31972747, 32172937 and 31772698) and Natural Science Foundation of Heilongjiang Province of China (YQ2019C014).