Goose IRF7 is involved in antivirus innate immunity by mediating IFN activation

Zhenyu Lin; Jie Wang; Shurui Zhao; Yanlin Li; Yanhe Zhang; Yue Wang; Yaxian Yan; Yuqiang Cheng; Jianhe Sun

doi:10.1016/j.dci.2022.104435

Goose IRF7 is involved in antivirus innate immunity by mediating IFN activation

Dev Comp Immunol. 2022 Aug:133:104435. doi: 10.1016/j.dci.2022.104435. Epub 2022 May 11.

Authors

Zhenyu Lin¹, Jie Wang¹, Shurui Zhao¹, Yanlin Li¹, Yanhe Zhang², Yue Wang², Yaxian Yan¹, Yuqiang Cheng³, Jianhe Sun⁴

Affiliations

¹ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Agriculture Ministry Key Laboratory of Urban Agriculture (South), Shanghai, 200240, China.
² State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
³ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Agriculture Ministry Key Laboratory of Urban Agriculture (South), Shanghai, 200240, China. Electronic address: wyycyq@sjtu.edu.cn.
⁴ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Agriculture Ministry Key Laboratory of Urban Agriculture (South), Shanghai, 200240, China. Electronic address: sunjhe@sjtu.edu.cn.

PMID: 35562079
DOI: 10.1016/j.dci.2022.104435

Abstract

Interferon regulatory factor (IRF) 3 and IRF7 are the most important nuclear transcription factors regulating type-I interferon (IFN) production in mammals and the IRF3 is missing in birds. Our previous study found that IFR7 is the most important IRF in chickens, however, its functions in geese remain unknown. We cloned goose IRF7 (GoIRF7) and conducted bioinformatics analyses to compare the chromosomal location and protein homology of IRF7 in different species. Overexpression of GoIRF7 in DF-1 cells induced the activation of IFN-β, and this activation correlated positively with the dosage of transfected plasmids. Overexpression of GoIRF7 in goose embryonic fibroblasts (GEFs) induced the expression of IFNs, proinflammatory cytokines, and IFN-stimulated genes (ISGs); it also inhibited replication of Newcastle disease virus (NDV) and vesicular stomatitis virus (VSV). Our results suggest that GoIRF7 is an important regulator of IFNs, proinflammatory cytokines, and ISGs and plays a role in antiviral innate immunity in geese.

Keywords: Goose; Innate immunity; Interferon; Interferon regulatory factor 7; Newcastle disease virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens*
Cytokines
Geese*
Immunity, Innate / genetics
Interferon Regulatory Factor-7 / genetics
Mammals
Newcastle disease virus
Virus Replication

Substances

Cytokines
Interferon Regulatory Factor-7