A novel gas production bioassay of thiosulfate utilizing denitrifying bacteria (TUDB) for the toxicity assessment of heavy metals contaminated water

Abstract

This study reports for the first-time the possibility of deploying gas production by thiosulfate utilizing denitrifying bacteria (TUDB) as a proxy to evaluate water toxicity. The test relies on gas production by TUDB due to inhibited metabolic activity in the presence of toxicants. Gas production was measured using a bubble-type respirometer. Optimization studies indicated that 300 mg NO₃^--N/L, 0.5 mL acclimated culture, and 2100 mg S₂O₃^2-/L were the ideal conditions facilitating the necessary volume of gas production for sensitive data generation. Determined EC₅₀ values of the selected heavy metals were: Cr⁶⁺, 0.51 mg/L; Ag⁺, 2.90 mg/L; Cu²⁺, 2.90 mg/L; Ni²⁺, 3.60 mg/L; As³⁺, 4.10 mg/L; Cd²⁺, 5.56 mg/L; Hg²⁺, 8.06 mg/L; and Pb²⁺, 19.3 mg/L. The advantages of this method include operational simplicity through the elimination of cumbersome preprocessing procedures which are used to eliminate interferences caused by turbidity when the toxicity of turbid samples is determined via spectrophotometry.

Keywords: Autotrophic denitrification; Bioassay; Gas production; Heavy metals; Thiosulfate; Water toxicity.