The potassium channel GhAKT2bD is regulated by CBL-CIPK calcium signalling complexes and facilitates K+ allocation in cotton

Abstract

Efficient allocation of the essential nutrient potassium (K⁺ ) is a central determinant of plant ion homeostasis and involves AKT2 K⁺ channels. Here, we characterize four AKT2 K⁺ channels from cotton and report that xylem and phloem expressed GhAKT2bD facilitates K⁺ allocation and that AKT2-silencing impairs plant growth and development. We uncover kinase activity-dependent activation of GhAKT2bD-mediated K⁺ uptake by AtCBL4-GhCIPK1 calcium signalling complexes in HEK293T cells. Moreover, AtCBL4-AtCIPK6 complexes known to convey activation of AtAKT2 in Arabidopsis also activate cotton GhAKT2bD in HEK293T cells. Collectively, these findings reveal an essential role for AKT2 in the source-sink allocation of K⁺ in cotton and identify GhAKT2bD as subject to complex regulation by CBL-CIPK Ca²⁺ sensor-kinase complexes.

Keywords: AKT2; CBL; CIPK; K+ channel; cotton; potassium allocation.