Phylogeny, Evolution, and Transmission Dynamics of Canine and Feline Coronaviruses: A Retro-Prospective Study

Hu Yang; Qianling Peng; Yifei Lang; SenYan Du; SanJie Cao; Rui Wu; Qin Zhao; Xiaobo Huang; Yiping Wen; Juchun Lin; Shan Zhao; Qigui Yan

doi:10.3389/fmicb.2022.850516

Phylogeny, Evolution, and Transmission Dynamics of Canine and Feline Coronaviruses: A Retro-Prospective Study

Front Microbiol. 2022 Apr 26:13:850516. doi: 10.3389/fmicb.2022.850516. eCollection 2022.

Authors

Hu Yang^{1

2}, Qianling Peng^{1

2}, Yifei Lang^{1

2}, SenYan Du^{1

2}, SanJie Cao^{1

2}, Rui Wu^{1

2}, Qin Zhao^{1

2}, Xiaobo Huang^{1

2}, Yiping Wen^{1

2}, Juchun Lin^{2

3}, Shan Zhao^{1

2}, Qigui Yan^{1

2}

Affiliations

¹ Swine Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
² Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
³ Department of Basic Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Abstract

Canine coronavirus (CCoV) and feline coronavirus (FCoV) are endemic in companion animals. Due to their high mutation rates and tendencies of genome recombination, they pose potential threats to public health. The molecular characteristics and genetic variation of both CCoV and FCoV have been thoroughly studied, but their origin and evolutionary dynamics still require further assessment. In the present study, we applied a comprehensive approach and analyzed the S, M, and N genes of different CCoV/FCoV isolates. Discriminant analysis of principal components (DAPC) and phylogenetic analysis showed that the FCoV sequences from Chinese isolates were closely related to the FCoV clusters in Netherlands, while recombination analysis indicated that of S N-terminal domain (NTD) was the most susceptible region of mutation, and recombination of this region is an important cause of the emergence of new lineages. Natural selection showed that CCoV and FCoV subgenotypes were in selection constraints, and CCoV-IIb was in strong positive selection. Phylodynamics showed that the mean evolution rate of S1 genes of CCoV and FCoV was 1.281 × 10^-3 and 1.244 × 10^-3 subs/site/year, respectively, and the tMRCA of CCoV and FCoV was about 1901 and 1822, respectively. Taken together, our study centered on tracing the origin of CCoV/FCoV and provided ample insights into the phylogeny and evolution of canine and feline coronaviruses.

Keywords: BEAST; DAPC; canine coronavirus; feline coronavirus; phylodynamics; phylogeny; recombination.