Methods of SARS-CoV-2 Inactivation

Enyia R Anderson; Tessa Prince; Lance Turtle; Grant L Hughes; Edward I Patterson

doi:10.1007/978-1-0716-2111-0_25

Methods of SARS-CoV-2 Inactivation

Methods Mol Biol. 2022:2452:465-473. doi: 10.1007/978-1-0716-2111-0_25.

Authors

Enyia R Anderson^{1

2

3}, Tessa Prince⁴, Lance Turtle^{4

5}, Grant L Hughes^#^{1

2

3}, Edward I Patterson^#⁶

Affiliations

¹ Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
² Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
³ Centre for Neglected Tropical Disease, Liverpool School of Tropical Medicine, Liverpool, UK.
⁴ NIHR Health Protection Unit in Emerging and Zoonotic Infections, Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, UK.
⁵ Tropical and Infectious Disease Unit, Liverpool University Hospitals Foundation NHS Trust (member of Liverpool Health Partners), Liverpool, UK.
⁶ Department of Biological Sciences, Brock University, St. Catharines, Canada. ipatterson@brocku.ca.

^# Contributed equally.

PMID: 35554921
DOI: 10.1007/978-1-0716-2111-0_25

Abstract

Inactivation methods allow for hazard group 3 (HG3) pathogens to be disposed of and used safely in downstream experiments and assays to be carried out at lower containment levels. Commonly used viral inactivation methods include heat inactivation, fixation methods, ultraviolet (UV) light and detergent inactivation. Here we describe known methods used to inactivate SARS-CoV-2 for safe downstream biological assays.

Keywords: Assay; Inactivation; SARS-CoV-2; TRIzol; UV; Vero.

MeSH terms

Animals
COVID-19*
Chlorocebus aethiops
SARS-CoV-2*
Ultraviolet Rays
Vero Cells
Virus Inactivation