Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test

Sandra Westhaus; Holger F Rabenau

doi:10.1007/978-1-0716-2111-0_20

Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test

Methods Mol Biol. 2022:2452:353-360. doi: 10.1007/978-1-0716-2111-0_20.

Authors

Sandra Westhaus¹, Holger F Rabenau²

Affiliations

¹ Institue of Medical Viroogy, University of Frankfurt, Goethe-University, Frankfurt, Germany. Sandra.Westhaus@uk-essen.de.
² Institue of Medical Viroogy, University of Frankfurt, Goethe-University, Frankfurt, Germany.

PMID: 35554916
DOI: 10.1007/978-1-0716-2111-0_20

Abstract

Neutralization assays are often used as part of research and diagnostics to detect neutralizing antibodies and to determine a possible protective antibody titer after infection or vaccination. Here we describe a conventional plaque reduction neutralization test (PRNT) to check the presence of antibodies against SARS-CoV-2 in patient samples (serum or plasma).

Keywords: Caco-2 cell line; Neutralizing antibody; Neutralizing assay; Plaque reduction neutralization test; SARS-CoV-2.

MeSH terms

Antibodies, Neutralizing
Antibodies, Viral
COVID-19* / diagnosis
Humans
Neutralization Tests
SARS-CoV-2

Substances

Antibodies, Neutralizing
Antibodies, Viral