Animal Models of COVID-19: Nonhuman Primates

Dhiraj K Singh; Journey Cole; Ruby A Escobedo; Kendra J Alfson; Bindu Singh; Tae-Hyung Lee; Xavier Alvarez; Shashank R Ganatra; Ricardo Carrion Jr; Deepak Kaushal

doi:10.1007/978-1-0716-2111-0_15

Animal Models of COVID-19: Nonhuman Primates

Methods Mol Biol. 2022:2452:227-258. doi: 10.1007/978-1-0716-2111-0_15.

Authors

Dhiraj K Singh^{1

2}, Journey Cole^{1

2}, Ruby A Escobedo^{1

2}, Kendra J Alfson², Bindu Singh^{1

2}, Tae-Hyung Lee^{1

2}, Xavier Alvarez^{1

2}, Shashank R Ganatra^{1

2}, Ricardo Carrion Jr², Deepak Kaushal^{3

4}

Affiliations

¹ Southwest National Primate Research Center, San Antonio, TX, USA.
² Texas Biomedical Research Institute, San Antonio, TX, USA.
³ Southwest National Primate Research Center, San Antonio, TX, USA. dkaushal@txbiomed.org.
⁴ Texas Biomedical Research Institute, San Antonio, TX, USA. dkaushal@txbiomed.org.

PMID: 35554911
DOI: 10.1007/978-1-0716-2111-0_15

Abstract

With the advent of the novel SARS-CoV-2, the entire world has been thrown into chaos with severe disruptions from a normal life. While the entire world was going chaotic, the researchers throughout the world were struggling to contribute to the best of their capabilities to advance the understanding of this new pandemic and fast track the development of novel therapeutics and vaccines. While various animal models have helped a lot to understand the basic physiology, nonhman primates have been promising and much more successful in modelling human diseases compared to other available clinical models. Here we describe the different aspects of modelling the SARS-CoV-2 infection in NHPs along with the associated methods used in NHP immunology.

Keywords: Animal models; BAL; COVID-19; Computed tomography; Cone beam; Confocal microscopy; FACS; Flow cytometry; IHC; In vivo imaging; NHP; Nonhuman primates; Rhesus macaques; SARS-CoV-2; X-rays.

MeSH terms

Animals
COVID-19*
Disease Models, Animal
Pandemics
Primates
SARS-CoV-2