Phosphatase-like activity of single-atom CeNC nanozyme for rapid detection of Al3

Guangchun Song; Jin-Cheng Li; Zainabu Majid; Wentao Xu; Xiaoyun He; Zhiyi Yao; Yunbo Luo; Kunlun Huang; Nan Cheng

doi:10.1016/j.foodchem.2022.133127

Phosphatase-like activity of single-atom CeNC nanozyme for rapid detection of Al³

Food Chem. 2022 Oct 1:390:133127. doi: 10.1016/j.foodchem.2022.133127. Epub 2022 May 5.

Authors

Guangchun Song¹, Jin-Cheng Li², Zainabu Majid¹, Wentao Xu³, Xiaoyun He¹, Zhiyi Yao¹, Yunbo Luo¹, Kunlun Huang⁴, Nan Cheng⁵

Affiliations

¹ Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
² Faculty of Chemical Engineering, Yunnan Provincial Key Laboratory of Energy Saving in Phosphorus, Chemical Engineering and New Phosphorus Materials, Kunming University of Science and Technology, Kunming 650000, China.
³ Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, Department of Nutrition and Health, China Agricultural University, Beijing 100191, China.
⁴ Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, Beijing 100083, China.
⁵ Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: chengnanFSNE@cau.edu.cn.

PMID: 35551022
DOI: 10.1016/j.foodchem.2022.133127

Abstract

Single-atom nanozymes are a class of nanozymes with attractive enzyme-like activities. They usually mimic oxidoreductases and lack other types of enzyme-like activities. Hence, we verified a single-atom CeNC (SACeNC) nanozyme with an excellent phosphatase-like (PPA-like) activity, which could catalyze the dephosphorylation of inorganic phosphates. Meanwhile, we found that Al³⁺ could specifically combine with the O atom in its structure to form an Al-O bond, which could inhibit its PPA-like activity. Based on this principle, we have constructed a fast, portable, and efficient fluorescent liquid phase sensor to detect Al³⁺. The detection time was only 4 min, and the limit of detection (LOD) was 22.89 ng/mL in the linear range of 5-25 μg/mL. This study not only verified that single-atom nanozymes mimic phosphatase activities, but also applied its unique enzyme-like to the field of food safety rapid detection.

Keywords: Al(3+) detection; Food safety; Phosphatase-like; Single atom CeNC nanozyme.

MeSH terms

Aluminum / analysis*
Catalysis
Limit of Detection
Oxidoreductases*
Phosphates
Phosphoric Monoester Hydrolases*

Substances

Phosphates
Aluminum
Oxidoreductases
Phosphoric Monoester Hydrolases