Sulfated capsular polysaccharide from the marine bacterium Kangiella japonica inhibits T-47D cells growth in vitro

Maxim S Kokoulin; Alexandra S Kuzmich; Lyudmila A Romanenko; Irina V Chikalovets

doi:10.1016/j.carbpol.2022.119477

Sulfated capsular polysaccharide from the marine bacterium Kangiella japonica inhibits T-47D cells growth in vitro

Carbohydr Polym. 2022 Aug 15:290:119477. doi: 10.1016/j.carbpol.2022.119477. Epub 2022 Apr 11.

Authors

Maxim S Kokoulin¹, Alexandra S Kuzmich², Lyudmila A Romanenko², Irina V Chikalovets³

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia. Electronic address: maxchem@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia; Far Eastern Federal University, 8, Sukhanova str., Vladivostok 690950, Russia.

PMID: 35550752
DOI: 10.1016/j.carbpol.2022.119477

Abstract

Kangiella japonica KMM 3897 is a Gram-negative bacterium isolated from a coastal sea-water sample of the Sea of Japan. In this paper, the results about the structure and the antiproliferative effect on cancer cells of the capsular polysaccharide isolated from the Kangiella japonica KMM 3897 have been described. The carbohydrate polymer was isolated and purified by several separation techniques, and the structure was elucidated using chemical analysis and NMR spectroscopy. The following structure of the sulfated capsular polysaccharide, containing 2-amino-2-deoxy-D-mannuronic acid was established: The capsular polysaccharide exerted a selective antiproliferative effect and suppressed the colony formation of T-47D cells.

Keywords: Antiproliferative activity; Breast cancer; Capsular polysaccharide; Kangiella japonica; Marine bacterium; Sulfate; T-47D.

MeSH terms

Cell Proliferation
Gammaproteobacteria*
Polysaccharides / pharmacology
Sulfates* / chemistry
Sulfates* / pharmacology

Substances

Polysaccharides
Sulfates

Supplementary concepts

Kangiella japonica