Accumulated oxidative stress risk in HUVECs by chronic exposure to non-observable acute effect levels of PM2.5

Bingru Nan; Xia Sun; Jie Zhang; Qingyu Huang; Xi Zhang; Yanbo Li; Junchao Duan; Rui Chen; Zhiwei Sun; Heqing Shen

doi:10.1016/j.tiv.2022.105376

Accumulated oxidative stress risk in HUVECs by chronic exposure to non-observable acute effect levels of PM_2.5

Toxicol In Vitro. 2022 Aug:82:105376. doi: 10.1016/j.tiv.2022.105376. Epub 2022 May 10.

Authors

Bingru Nan¹, Xia Sun², Jie Zhang³, Qingyu Huang⁴, Xi Zhang⁵, Yanbo Li⁶, Junchao Duan⁶, Rui Chen⁶, Zhiwei Sun⁶, Heqing Shen⁷

Affiliations

¹ Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
² Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou, Fujian 350108, China.
³ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, PR China.
⁴ Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China.
⁵ School of Medicine, Ningbo University, Ningbo 315211, China.
⁶ Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China.
⁷ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, PR China; Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen 361003, PR China. Electronic address: hqshen@xmu.edu.cn.

PMID: 35550414
DOI: 10.1016/j.tiv.2022.105376

Abstract

Few studies have reported the accumulation of non-observable acute effect (NOAE) of PM_2.5, especially exposure to the NOAE doses (NOAEDs) of PM_2.5 in chronic way. To address this issue, HUVECs were cultured from the 1st to 30th generations (G1 to G30) and treated by the NOAED PM_2.5 once every three passages. The generational changes of oxidative damage markers, inflammatory factors, and cell adhesion molecules (CAMs) were monitored in HUVECs at G6, G12, G18, G24, and G30, and proteomes at G18 and G30, respectively. The oxidative damages monotonically accumulated with exposure time elongation and PM_2.5 dose increases. Similar to the oxidative trends, VCAM1 and ICAM1 significantly and dose-dependently increased at G30. However, many inflammatory factors altered with complex patterns to respond the NOAEDs' PM_2.5. Proteomic results demonstrated most proteins expressed stably, and the generational proteome alterations were more apparent than the NOAEDs' PM_2.5 induced ones. The PM_2.5-related proteins varied much, but only few can cross the doses and generations. These observations suggested that the proteins changed holistically rather than individually. In summary, SOD1, SUMO2, and H3F3A may initiate HUVECs responses to PM_2.5, and then broadcast and accumulate the NOAE via DNA repair, immune response, and glycolysis.

Keywords: HUVECs; Non-observable acute effect dose; Oxidative stress; PM(2.5); Proteomics.

MeSH terms

Air Pollutants* / toxicity
Oxidation-Reduction
Oxidative Stress
Particulate Matter* / toxicity
Proteomics

Substances

Air Pollutants
Particulate Matter