Bletilla striata Oligosaccharides Improve Ulcerative Colitis by Regulating Gut Microbiota and Intestinal Metabolites in Dextran Sulfate Sodium-Induced Mice

Tianxiang Zhu; Baifei Hu; Cheng Ye; Haiming Hu; Mingzhu Yin; Zhigang Zhang; Shuiqing Li; Yanju Liu; Hongtao Liu

doi:10.3389/fphar.2022.867525

Bletilla striata Oligosaccharides Improve Ulcerative Colitis by Regulating Gut Microbiota and Intestinal Metabolites in Dextran Sulfate Sodium-Induced Mice

Front Pharmacol. 2022 Apr 25:13:867525. doi: 10.3389/fphar.2022.867525. eCollection 2022.

Authors

Tianxiang Zhu¹, Baifei Hu¹, Cheng Ye², Haiming Hu¹, Mingzhu Yin¹, Zhigang Zhang¹, Shuiqing Li¹, Yanju Liu¹, Hongtao Liu¹

Affiliations

¹ College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan, China.
² Wuhan Customs Technology Center, Wuhan, China.

Abstract

This study aimed to elucidate the mechanism of Bletilla striata oligosaccharides (BO) in the treatment of ulcerative colitis (UC). A UC mouse model was induced by 3% Dextran sodium sulfate (DSS), and BO (200 mg/kg/d) were administered for intervention. The results show that BO effectively inhibited the release of intestinal inflammatory cytokines such as IL-6, TNF-α, and IL-1β. Also, BO profoundly elevated the secretion of mucins and the expression of tight junction (TJ) proteins to attenuate dysfunction of the intestinal barrier. The 16S rDNA sequencing and liquid chromatography/gas chromatography-mass spectrometer (LC/GC-MS) analysis of mouse feces revealed that BO regulated the disturbance of gut microbiota and intestinal metabolites. By using the in vitro fermentation broth of BO and gut microbiota-depleted mice treated with antibiotics, we confirmed the protection of BO against UC. In conclusion, BO played a role in improving UC by modulating gut microbial composition and intestinal metabolites, which provided new therapeutic strategies for UC treatment.

Keywords: Bletilla striata oligosaccharides; gut microbiota; intestinal barrier; intestinal metabolites; ulcerative colitis.