Three new Penicillium species isolated from the tidal flats of China

Ke-Xin Xu; Xia-Nan Shan; Yongming Ruan; JianXin Deng; Long Wang

doi:10.7717/peerj.13224

Three new Penicillium species isolated from the tidal flats of China

PeerJ. 2022 May 6:10:e13224. doi: 10.7717/peerj.13224. eCollection 2022.

Authors

Ke-Xin Xu^#¹, Xia-Nan Shan^#², Yongming Ruan², JianXin Deng¹, Long Wang³

Affiliations

¹ College of Agriculture, Yangtze University, Jingzhou, Hubei, China.
² College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, Zhejiang, China.
³ State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

^# Contributed equally.

Abstract

During a survey of culturable fungi in the coastal areas of China, three new species of Penicillium sect. Lanata-Divaricata were discovered and studied with a polyphasic taxonomic approach, and then named as P. donggangicum sp. nov. (ex-type AS3.15900^T = LN5H1-4), P. hepuense sp. nov. (ex-type AS3.16039^T = TT2-4X3, AS3.16040 = TT2-6X3) and P. jiaozhouwanicum sp. nov. (ex-type AS3.16038^T = 0801H2-2, AS3.16207 = ZZ2-9-3). In morphology, P. donggangicum is unique in showing light yellow sclerotia and mycelium, sparse sporulation, restricted growth at 37 °C, irregular conidiophores, intercalary phialides and metulae, and pyriform to subspherical conidia. P. hepuense is distinguished by the fast growth on CYA and YES and slow growth on MEA at 25 °C, weak or absence of growth at 37 °C, biverticillate and monoverticillate penicilli, and ellipsoidal conidia. P. jiaozhouwanicum is characterized by abundant grayish-green conidia en masse and moderate growth at 37 °C, the appressed biverticillate penicilli and fusiform, smooth-walled conidia. These three novelties were further confirmed by the phylogenetic analyses based on either the combined BenA-CaM-Rpb2 or the individual BenA, CaM, Rpb2 and internal transcribed spacer (ITS) sequences.

Keywords: Mudflats; Mycobiota; New taxa; Penicillia; Polyphasic taxonomy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
DNA, Fungal / genetics
DNA, Ribosomal Spacer
Penicillium* / genetics
Phylogeny
Spores, Fungal

Substances

DNA, Ribosomal Spacer
DNA, Fungal

Grants and funding

This work was supported by the National Natural Science Foundation of China (U20A20101) and the National Project on Scientific Groundwork, Ministry of Science and Technology of China (2019FY100700). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.