Janus colloidal motors (JCMs) are versatile anisotropic particles that can effectively move autonomously based on their asymmetric structures, providing unlimited possibilities for various tasks. Developing novel JCMs with controllable size, engineered nanostructure and functionalized surface properties has always been a challenge for chemists. This review summarizes the recent progress in synthesized JCMs in terms of their fabrication method, propulsion strategy, and biomedical applications. The design options, construction methods, and typical examples of JCMs are presented. Common propulsion mechanisms of JCMs are reviewed, as well as the approaches to control their motion under complex microscopic conditions based on symmetry-breaking strategies. The precisely controlled motion enables JCMs to be used in biomedicine, environmental remediation, analytical sensing and nanoengineering. Finally, perspectives on future research and development are presented. Through ingenious design and multi-functionality, new JCM-based technologies could address more and more special needs in complex environments.