Nanomaterial-Embedded DNA Films on 2D Frames

Karthikeyan Mariyappan; Anshula Tandon; Suyoun Park; Samanth Kokkiligadda; Jayeon Lee; Soojin Jo; Eswaravara Prasadarao Komarala; Sanghyun Yoo; Prathamesh Chopade; Hee Jin Choi; Chang-Won Lee; Sohee Jeon; Jun-Ho Jeong; Sung Ha Park

doi:10.1021/acsabm.2c00227

Nanomaterial-Embedded DNA Films on 2D Frames

ACS Appl Bio Mater. 2022 Jun 20;5(6):2812-2818. doi: 10.1021/acsabm.2c00227. Epub 2022 May 11.

Authors

Affiliations

¹ Department of Physics, Institute of Basic Science, and Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 16419, Korea.
² Institute of Advanced Optics and Photonics, Department of Applied Optics, Hanbat National University, Daejeon 34158, Korea.
³ Nanomechanical Systems Research Division, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Korea.
⁴ Department of Nanomechatronics, Korea University of Science and Technology (UST), Daejeon 34113, Korea.

PMID: 35543024
DOI: 10.1021/acsabm.2c00227

Abstract

Recently, 3D printing has provided opportunities for designing complex structures with ease. These printed structures can serve as molds for complex materials such as DNA and cetyltrimethylammonium chloride (CTMA)-modified DNA that have easily tunable functionalities via the embedding of various nanomaterials such as ions, nanoparticles, fluorophores, and proteins. Herein, we develop a simple and efficient method for constructing DNA flat and curved films containing water-soluble/thermochromatic dyes and di/trivalent ions and CTMA-modified DNA films embedded with organic light-emitting molecules (OLEM) with the aid of 2D/3D frames made by a 3D printer. We study the Raman spectra, current, and resistance of Cu²⁺-doped and Tb³⁺-doped DNA films and the photoluminescence of OLEM-embedded CTMA-modified DNA films to better understand the optoelectric characteristics of the samples. Compared to pristine DNA, ion-doped DNA films show noticeable variation of Raman peak intensities, which might be due to the interaction between the ion and phosphate backbone of DNA and the intercalation of ions in DNA base pairs. As expected, ion-doped DNA films show an increase of current with an increase in bias voltage. Because of the presence of metallic ions, DNA films with embedded ions showed relatively larger current than pristine DNA. The photoluminescent emission peaks of CTMA-modified DNA films with OLEM_Red, OLEM_Green, and OLEM_Blue were obtained at the wavelengths of 610, 515, and 469 nm, respectively. Finally, CIE color coordinates produced from CTMA-modified DNA films with different OLEM color types were plotted in color space. It may be feasible to produce multilayered DNA films as well. If so, multilayered DNA films embedded with different color dyes, ions, fluorescent materials, nanoparticles, proteins, and drug molecules could be used to realize multifunctional physical devices such as energy harvesting and chemo-bio sensors in the near future.

Keywords: 3D printer; DNA film; frame; optoelectrical characteristics; photoluminescence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cetrimonium
Coloring Agents
DNA* / chemistry
Ions
Nanostructures* / chemistry

Substances

Coloring Agents
Ions
DNA
Cetrimonium