A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7

Yulin Wu; Jun Liu; Huili Hao; Lianmei Hu; Xiaoyong Zhang; Lianxiang Luo; Jincheng Zeng; Wei Zhang; Io Nam Wong; Riming Huang

doi:10.1016/j.fochx.2022.100313

A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7

Food Chem X. 2022 Apr 19:14:100313. doi: 10.1016/j.fochx.2022.100313. eCollection 2022 Jun 30.

Authors

Yulin Wu¹, Jun Liu², Huili Hao¹, Lianmei Hu³, Xiaoyong Zhang⁴, Lianxiang Luo², Jincheng Zeng², Wei Zhang⁵, Io Nam Wong⁵, Riming Huang¹

Affiliations

¹ Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
² Laboratory of Pathogenic Biology, The Marine Biomedical Research Institute, Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
³ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
⁴ Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.
⁵ State Key Laboratory of Quality Research in Chinese Medicines and Macau Institute for Applied Research in Medicine and Health, Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau 999078, China.

Abstract

Investigation on Caulerpa chemnitzia polysaccharides led to the finding of a new polysaccharide (CCP). The basic components of CCP were the total sugar (59.18% ± 0.57%), the uronic acids (36.75% ± 0.28%) and the sulfate (42.50% ± 0.42%), in total content. The physicochemical analysis revealed that CCP was a heteropolysaccharide with a molecular weight of 321.6 KDa, and composed of arabinose, fucose, glucose, mannose, galactose, xylose, fructose, ribose, glucuronic acid and galacturonic acid. The immunomodulatory assay showed that CCP played an important role in activating cell viability, the nitric oxide product and cytokines (IL-6 and TNF-α) secretion. Furthermore, the transcript-metabolic analysis displayed a total of 7692 differentially expressed genes (DEGs) and 95 differentially accumulated metabolites (DAMs), and revealed that CCP may play an immunomodulatory effect by activating NF-κB signaling pathway and arachidonic acid metabolism pathway. These findings will provide a basic understanding to further investigation of Caulerpa polysaccharides.

Keywords: Arachidonic acid metabolism pathway; Caulerpa chemnitzia; Immunomodulatory effect; NF-κB signaling pathway; Polysaccharide; Transcript-metabolic analysis.